Site-Specific Delivery of Doxorubicin Using Cell-Penetrating Peptide for Lung Cancer Chemotherapy

Recent studies have focused heavily on tumor-oriented nanocarriers mediated by cellpenetrating peptides (CPPs). However, the loss of CPPs in normal tissues and enzymatic degradation circulation frequently prevented use vivo. To alleviate these limitations, needed to be kept immobilized before they arrived at intended target for receptor-mediated endocytosis (RME). In this study, we developed CAR/DOX-Liposomes with doxorubicin hydrochloride (DOX) entrapped hydrophilic core liposomes using a thin film hydration method, CAR peptide was subsequently conjugated through SPDP chemistry surface as targeting moiety develop improved targeted cancer chemotherapy. The prepared were characterized evaluated different parameters which recorded vesicle size 275.2±5.65 nm, polydispersity index 0.260±0.85, Zeta-potential -33.90±2.42 mV, % entrapment efficiency 83.96±2.56 %. addition, Transmission electron microscopy (TEM), Atomic Force Microscopy (AFM) conducted assess morphology vitro drug release performed. Further, Cell line study studied over lung cell HOP-62 comparison IC50 values determined. establishes that offer specific delivery DOX heparan sulfate receptor(s) exclusively overexpressed cells.